Mahshid SS, Higazi AM, Ogier JM, Dabdoub A. Extracellular biomarkers of inner ear disease and their potential for point-of-care diagnostics. Adv Sci (Weinh). 2022;9:e2104033.
Chadha S, Kamenov K, Cieza A. The world report on hearing, 2021. Bull World Health Organ. 2021;99:242–A242.
Su YT, Guo YB, Cheng YP, Zhang X, Xie XP, Chang YM, Bao JX. Hyperbaric oxygen treatment ameliorates hearing loss and auditory cortex injury in noise exposed mice by repressing local ceramide accumulation. Int J Mol Sci. 2019;20.
Brock PR, Maibach R, Childs M, Rajput K, Roebuck D, Sullivan MJ, Laithier V, Ronghe M, Dall’Igna P, Hiyama E, et al. Sodium thiosulfate for protection from cisplatin-induced hearing loss. N Engl J Med. 2018;378:2376–85.
Zhang Y, Fang Q, Wang H, Qi J, Sun S, Liao M, Wu Y, Hu Y, Jiang P, Cheng C, et al. Increased mitophagy protects cochlear hair cells from aminoglycoside-induced damage. Autophagy. 2023;19:75–91.
Tian L, He L, Jackson K, Saif A, Khan S, Wan Z, Didar TF, Hosseinidoust Z. Self-assembling nanofibrous bacteriophage microgels as sprayable antimicrobials targeting multidrug-resistant bacteria. Nat Commun. 2022;13:7158.
Tian L, Jackson K, He L, Khan S, Thirugnanasampanthar M, Gomez M, Bayat F, Didar TF, Hosseinidoust Z. High-throughput fabrication of antimicrobial phage microgels and example applications in food decontamination. Nat Protoc. 2024;19:1591–622.
Korver AM, Smith RJ, Van Camp G, Schleiss MR, Bitner-Glindzicz MA, Lustig LR, Usami SI, Boudewyns AN. Congenital hearing loss. Nat Rev Dis Primers. 2017;3:16094.
Nyberg S, Abbott NJ, Shi X, Steyger PS, Dabdoub A. Delivery of therapeutics to the inner ear: the challenge of the blood-labyrinth barrier. Sci Transl Med. 2019;11.
Fritzsch B, Silos-Santiago I, Bianchi LM, Fariñas I. The role of neurotrophic factors in regulating the development of inner ear innervation. Trends Neurosci. 1997;20:159–64.
Li L, Luo J, Lin X, Tan J, Li P. Nanomaterials for inner ear diseases: challenges, limitations and opportunities. Mater (Basel). 2022;15.
Zhang H, Chen H, Lu L, Wang H, Zhao Y, Chai R. Natural multifunctional silk microcarriers for noise-induced hearing loss therapy. Adv Sci (Weinh). 2024;11:e2305215.
Takeda H, Dondzillo A, Randall JA, Gubbels SP. Challenges in cell-based therapies for the treatment of hearing loss. Trends Neurosci. 2018;41:823–37.
Pan X, Li Y, Huang P, Staecker H, He M. Extracellular vesicles for developing targeted hearing loss therapy. J Control Release. 2024;366:460–78.
Ma Y, Wise AK, Shepherd RK, Richardson RT. New molecular therapies for the treatment of hearing loss. Pharmacol Ther. 2019;200:190–209.
Jiang L, Wang D, He Y, Shu Y. Advances in gene therapy hold promise for treating hereditary hearing loss. Mol Ther. 2023;31:934–50.
Chen H, Sun L, Wang Y, Cai L, Zhao Y, Shang L. Biomimetic air purification with liquid-gating topological gradient microfluidics. Nat Chem Eng. 2024;1:650–60.
Shan J, Che J, Song C, Zhao Y. Emerging antibacterial nanozymes for wound healing. Smart Med. 2023;2:e20220025.
Tian L, He L, Jackson K, Mahabir R, Hosseinidoust Z. Bacteria repellent protein hydrogel decorated with tunable, isotropic, nano-on-micro hierarchical microbump array. Chem Commun (Camb). 2021;57:10883–6.
Zhang X, Lu M, Cao X, Zhao Y. Functional microneedles for wearable electronics. Smart Med. 2023;2:e20220023.
Swan EE, Mescher MJ, Sewell WF, Tao SL, Borenstein JT. Inner ear drug delivery for auditory applications. Adv Drug Deliv Rev. 2008;60:1583–99.
Koushik TM, Miller CM, Antunes E. Bone tissue engineering scaffolds: function of multi-material hierarchically structured scaffolds. Adv Healthc Mater. 2023;12:e2202766.
Glueckert R, Johnson Chacko L, Rask-Andersen H, Liu W, Handschuh S, Schrott-Fischer A. Anatomical basis of drug delivery to the inner ear. Hear Res. 2018;368:10–27.
Li L, Chao T, Brant J, O’Malley B Jr., Tsourkas A, Li D. Advances in nano-based inner ear delivery systems for the treatment of sensorineural hearing loss. Adv Drug Deliv Rev. 2017;108:2–12.
Liu S, Wang S, Zou L, Xiong W. Mechanisms in cochlear hair cell mechano-electrical transduction for acquisition of sound frequency and intensity. Cell Mol Life Sci. 2021;78:5083–94.
Driver EC, Kelley MW. Development of the cochlea. Development. 2020;147.
White HJ, Helwany M, Biknevicius AR, Peterson DC. Anatomy, Head and Neck, Ear Organ of Corti. In StatPearls. Treasure Island (FL) ineligible companies. Disclosure: Muhammad Helwany declares no relevant financial relationships with ineligible companies. Disclosure: Audrone Biknevicius declares no relevant financial relationships with ineligible companies. Disclosure: Diana Peterson declares no relevant financial relationships with ineligible companies.: StatPearls Publishing Copyright © 2025, StatPearls Publishing LLC.; 2025.
Dubrulle F, Chaton V, Risoud M, Farah H, Charley Q, Vincent C. The round window sign: a sensitive sign to detect perilymphatic fistulae on delayed postcontrast 3D-FLAIR sequence. Eur Radiol. 2020;30:6303–10.
Rathnam C, Chueng SD, Ying YM, Lee KB, Kwan K. Developments in bio-inspired nanomaterials for therapeutic delivery to treat hearing loss. Front Cell Neurosci. 2019;13:493.
Holley MC. Keynote review: the auditory system, hearing loss and potential targets for drug development. Drug Discov Today. 2005;10:1269–82.
Pararas EE, Borkholder DA, Borenstein JT. Microsystems technologies for drug delivery to the inner ear. Adv Drug Deliv Rev. 2012;64:1650–60.
Lynch ED, Kil J. Compounds for the prevention and treatment of noise-induced hearing loss. Drug Discov Today. 2005;10:1291–8.
Borenstein JT. Intracochlear drug delivery systems. Expert Opin Drug Deliv. 2011;8:1161–74.
Sendowski I, Abaamrane L, Raffin F, Cros A, Clarençon D. Therapeutic efficacy of intra-cochlear administration of methylprednisolone after acoustic trauma caused by gunshot noise in Guinea pigs. Hear Res. 2006;221:119–27.
Rybak LP. Mechanisms of cisplatin ototoxicity and progress in otoprotection. Curr Opin Otolaryngol Head Neck Surg. 2007;15:364–9.
Rybak LP, Whitworth CA. Ototoxicity: therapeutic opportunities. Drug Discov Today. 2005;10:1313–21.
Rybak LP, Ramkumar V. Ototoxicity. Kidney Int. 2007;72:931–5.
Rybak LP, Whitworth CA, Mukherjea D, Ramkumar V. Mechanisms of cisplatin-induced ototoxicity and prevention. Hear Res. 2007;226:157–67.
Autoimmune sensorineural hearing loss. Lancet. 1980;1:187.
Matteson EL, Fabry DA, Strome SE, Driscoll CL, Beatty CW, McDonald TJ. Autoimmune inner ear disease: diagnostic and therapeutic approaches in a multidisciplinary setting. J Am Acad Audiol. 2003;14:225–30.
Pathak S, Vambutas A. Autoimmune inner ear disease patient-associated 28-kDa proinflammatory IL-1β fragment results from caspase-7-mediated cleavage in vitro. JCI Insight. 2020;5.
Shamriz O, Tal Y, Gross M. Autoimmune inner ear disease: immune biomarkers, audiovestibular aspects, and therapeutic modalities of Cogan’s syndrome. J Immunol Res. 2018;2018:1498640.
Komune S, Snow JB Jr. Ototoxicity of Kanamycin sulfate and the barriers in the inner ear. Otolaryngol Head Neck Surg. 1981;89:1013–8.
Chen GD, Daszynski DM, Ding D, Jiang H, Woolman T, Blessing K, Kador PF, Salvi R. Novel oral multifunctional antioxidant prevents noise-induced hearing loss and hair cell loss. Hear Res. 2020;388:107880.
Wilson WR, Byl FM, Laird N. The efficacy of steroids in the treatment of idiopathic sudden hearing loss. A double-blind clinical study. Arch Otolaryngol. 1980;106:772–6.
Chandrasekhar SS, Tsai Do BS, Schwartz SR, Bontempo LJ, Faucett EA, Finestone SA, Hollingsworth DB, Kelley DM, Kmucha ST, Moonis G, et al. Clinical practice guideline: sudden hearing loss (Update). Otolaryngol Head Neck Surg. 2019;161:S1–45.
Graham MD, Sataloff RT, Kemink JL. Titration streptomycin therapy for bilateral Meniere’s disease: a preliminary report. Otolaryngol Head Neck Surg. 1984;92:440–7.
Sajjadi H, Paparella MM. Meniere’s disease. Lancet. 2008;372:406–14.
Balyan FR, Taibah A, De Donato G, Aslan A, Falcioni M, Russo A, Sanna M. Titration streptomycin therapy in Meniere’s disease: long-term results. Otolaryngol Head Neck Surg. 1998;118:261–6.
McCall AA, Swan EE, Borenstein JT, Sewell WF, Kujawa SG, McKenna MJ. Drug delivery for treatment of inner ear disease: current state of knowledge. Ear Hear. 2010;31:156–65.
Yang C, Yu Y, Shang L, Zhao Y. Flexible hemline-shaped microfibers for liquid transport. Nat Chem Eng. 2024;1:87–96.
Mirsalehi M, Ghajarzadeh M, Farhadi M, Akbarnejad Z, Ahmadi S, Salem MM. Intratympanic corticosteroid injection as a first-line treatment of the patients with idiopathic sudden sensorineural hearing loss compared to systemic steroid: a systematic review and meta-analysis. Am J Otolaryngol. 2022;43:103505.
Jackson LE, Silverstein H. Chemical perfusion of the inner ear. Otolaryngol Clin North Am. 2002;35:639–53.
Rauch SD, Halpin CF, Antonelli PJ, Babu S, Carey JP, Gantz BJ, Goebel JA, Hammerschlag PE, Harris JP, Isaacson B, et al. Oral vs intratympanic corticosteroid therapy for idiopathic sudden sensorineural hearing loss: a randomized trial. JAMA. 2011;305:2071–9.
Jung SY, Kim S, Kang Z, Kwon S, Lee J, Park JW, Kim KS, Kim DK. Efficiency of a dexamethasone nanosuspension as an intratympanic injection for acute hearing loss. Drug Deliv. 2022;29:149–60.
Zhang W, Cai L, Gan J, Zhao Y. Photothermal responsive porous hollow microneedles as Chinese medicine versatile delivery system for wound healing. Smart Med. 2024;3:e20240007.
Suryanarayanan R, Cook JA. Long-term results of gentamicin inner ear perfusion in Ménière’s disease. J Laryngol Otol. 2004;118:489–95.
Bowe SN, Jacob A. Round window perfusion dynamics: implications for intracochlear therapy. Curr Opin Otolaryngol Head Neck Surg. 2010;18:377–85.
Li L, Ren J, Yin T, Liu W. Intratympanic dexamethasone perfusion versus injection for treatment of refractory sudden sensorineural hearing loss. Eur Arch Otorhinolaryngol. 2013;270:861–7.
Hoffer ME, Kopke RD, Weisskopf P, Gottshall K, Allen K, Wester D. Microdose gentamicin administration via the round window microcatheter: results in patients with Meniere’s disease. Ann N Y Acad Sci. 2001;942:46–51.
Sale PJP, Uschakov A, Saief T, Rowe DP, Abbott CJ, Luu CD, Hampson AJ, O’Leary SJ, Sly DJ. Cannula-based drug delivery to the Guinea pig round window causes a lasting hearing loss that may be temporarily mitigated by BDNF. Hear Res. 2017;356:104–15.
Malfeld K, Baumhoff P, Volk HA, Lenarz T, Scheper V. Local long-term inner ear drug delivery in normal hearing guinea pig-an animal model to develop preventive treatment for noise-induced hearing loss. Biomolecules. 2022;12.
Schwieger J, Frisch AS, Rau TS, Lenarz T, Hügl S, Scheper V. 3D printed cell culture chamber for testing the effect of pump-based chronic drug delivery on inner ear tissue. Biomolecules. 2022;12.
Henry S, McAllister DV, Allen MG, Prausnitz MR. Microfabricated microneedles: a novel approach to transdermal drug delivery. J Pharm Sci. 1999;88:948.
Leong S, Aksit A, Feng SJ, Kysar JW, Lalwani AK. Inner ear diagnostics and drug delivery via microneedles. J Clin Med. 2022;11.
Aksit A, Rastogi S, Nadal ML, Parker AM, Lalwani AK, West AC, Kysar JW. Drug delivery device for the inner ear: ultra-sharp fully metallic microneedles. Drug Deliv Transl Res. 2021;11:214–26.
Luo X, Yang L, Cui Y. Microneedles: materials, fabrication, and biomedical applications. Biomed Microdevices. 2023;25:20.
Ahmed S, Al-Japairai K, Mahmood S, Hamed Almurisi S, Reddy Venugopal J, Rebhi Hilles A, Azmana M, Raman S. Current trends in polymer microneedle for transdermal drug delivery. Int J Pharm. 2020;587:119673.
Pawley DC, Goncalves S, Bas E, Dikici E, Deo SK, Daunert S, Telischi F. Dexamethasone (DXM)-coated poly(lactic-co-glycolic acid) (PLGA) microneedles as an improved drug delivery system for intracochlear biodegradable devices. Adv Ther. 2021;4:2100155.
Leong S, Aksit A, Szeto B, Feng SJ, Ji X, Soni RK, Olson ES, Kysar JW, Lalwani AK. Anatomic, physiologic, and proteomic consequences of repeated microneedle-mediated perforations of the round window membrane. Hear Res. 2023;432:108739.
Chikar JA, Hendricks JL, Richardson-Burns SM, Raphael Y, Pfingst BE, Martin DC. The use of a dual PEDOT and RGD-functionalized alginate hydrogel coating to provide sustained drug delivery and improved cochlear implant function. Biomaterials. 2012;33:1982–90.
Wang Y, Guo J, Cao X, Zhao Y. Developing conductive hydrogels for biomedical applications. Smart Med. 2024;3:e20230023.
Chen G, Wang F, Zhang X, Shang Y, Zhao Y. Living microecological hydrogels for wound healing. Sci Adv. 2023;9:eadg3478.
Coviello T, Matricardi P, Alhaique F. Drug delivery strategies using polysaccharidic gels. Expert Opin Drug Deliv. 2006;3:395–404.
Mano JF, Silva GA, Azevedo HS, Malafaya PB, Sousa RA, Silva SS, Boesel LF, Oliveira JM, Santos TC, Marques AP, et al. Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends. J R Soc Interface. 2007;4:999–1030.
Noushi F, Richardson RT, Hardman J, Clark G, O’Leary S. Delivery of neurotrophin-3 to the cochlea using alginate beads. Otol Neurotol. 2005;26:528–33.
Endo T, Nakagawa T, Kita T, Iguchi F, Kim TS, Tamura T, Iwai K, Tabata Y, Ito J. Novel strategy for treatment of inner ears using a biodegradable gel. Laryngoscope. 2005;115:2016–20.
Chen K, Wang F, Ding R, Cai Z, Zou T, Zhang A, Guo D, Ye B, Cui W, Xiang M. Adhesive and injectable hydrogel microspheres for inner ear treatment. Small. 2022;18:e2106591.
Wang J, Wang C, Wang Q, Zhang Z, Wang H, Wang S, Chi Z, Shang L, Wang W, Shu Y. Microfluidic preparation of gelatin methacryloyl microgels as local drug delivery vehicles for hearing loss therapy. ACS Appl Mater Interfaces. 2022;14:46212–23.
Feng B, Dong T, Song X, Zheng X, Jin C, Cheng Z, Liu Y, Zhang W, Wang X, Tao Y, Wu H. Personalized porous gelatin methacryloyl sustained-release nicotinamide protects against noise-induced hearing loss. Adv Sci (Weinh). 2024;11:e2305682.
Chen G, Zhang X, Yang F, Mu L. Disposition of nanoparticle-based delivery system via inner ear administration. Curr Drug Metab. 2010;11:886–97.
Huang D, Cai L, Li N, Zhao Y. Ultrasound-trigged micro/nanorobots for biomedical applications. Smart Med. 2023;2:e20230003.
Jaudoin C, Agnely F, Nguyen Y, Ferrary E, Bochot A. Nanocarriers for drug delivery to the inner ear: physicochemical key parameters, biodistribution, safety and efficacy. Int J Pharm. 2021;592:120038.
Zhao Z, Han Z, Naveena K, Lei G, Qiu S, Li X, Li T, Shi X, Zhuang W, Li Y, et al. ROS-responsive nanoparticle as a berberine carrier for OHC-targeted therapy of noise-induced hearing loss. ACS Appl Mater Interfaces. 2021;13:7102–14.
Wang Y, Wise AK, Tan J, Maina JW, Shepherd RK, Caruso F. Mesoporous silica supraparticles for sustained inner-ear drug delivery. Small. 2014;10:4244–8.
Xu K, Du Y, Xu B, Huang Y, Feng W, Yu D, Chen Y, Wang X. Gelatin-encapsulated tetrahedral DNA nanostructure enhances cellular internalization for treating noise-induced hearing loss. Small. 2024;20:e2310604.
Gao G, Liu Y, Zhou CH, Jiang P, Sun JJ. Solid lipid nanoparticles loaded with edaravone for inner ear protection after noise exposure. Chin Med J (Engl). 2015;128:203–9.
Martín-Saldaña S, Palao-Suay R, Aguilar MR, García-Fernández L, Arévalo H, Trinidad A, Ramírez-Camacho R. San Román J: pH-sensitive polymeric nanoparticles with antioxidant and anti-inflammatory properties against cisplatin-induced hearing loss. J Control Release. 2018;270:53–64.
Szeto B, Chiang H, Valentini C, Yu M, Kysar JW, Lalwani AK. Inner ear delivery: challenges and opportunities. Laryngoscope Investig Otolaryngol. 2020;5:122–31.
Hao J, Li SK. Inner ear drug delivery: recent advances, challenges, and perspective. Eur J Pharm Sci. 2019;126:82–92.
De Ceulaer G, Johnson S, Yperman M, Daemers K, Offeciers FE, O’Donoghue GM, Govaerts PJ. Long-term evaluation of the effect of intracochlear steroid deposition on electrode impedance in cochlear implant patients. Otol Neurotol. 2003;24:769–74.
Prenzler NK, Salcher R, Lenarz T, Gaertner L, Warnecke A. Dose-dependent transient decrease of impedances by deep intracochlear injection of triamcinolone with a cochlear catheter prior to cochlear implantation-1 year data. Front Neurol. 2020;11:258.
Macherey O, Carlyon RP. Cochlear implants. Curr Biol. 2014;24:R878–84.
Duan ML, Ulfendahl M, Laurell G, Counter SA, Pyykkö I, Borg E, Rosenhall U. Protection and treatment of sensorineural hearing disorders caused by exogenous factors: experimental findings and potential clinical application. Hear Res. 2002;169:169–78.
Astolfi L, Guaran V, Marchetti N, Olivetto E, Simoni E, Cavazzini A, Jolly C, Martini A. Cochlear implants and drug delivery: in vitro evaluation of dexamethasone release. J Biomed Mater Res B Appl Biomater. 2014;102:267–73.
Mukherjee S, Kuroiwa M, Oakden W, Paul BT, Noman A, Chen J, Lin V, Dimitrijevic A, Stanisz G, Le TN. Local magnetic delivery of adeno-associated virus AAV2(quad Y-F)-mediated BDNF gene therapy restores hearing after noise injury. Mol Ther. 2022;30:519–33.
Pinyon JL, Tadros SF, Froud KE, AC YW, Tompson IT, Crawford EN, Ko M, Morris R, Klugmann M, Housley GD. Close-field electroporation gene delivery using the cochlear implant electrode array enhances the bionic ear. Sci Transl Med. 2014;6:233ra254.
Duan YY, Clark GM, Cowan RS. A study of intra-cochlear electrodes and tissue interface by electrochemical impedance methods in vivo. Biomaterials. 2004;25:3813–28.
Richardson RT, Wise AK, Thompson BC, Flynn BO, Atkinson PJ, Fretwell NJ, Fallon JB, Wallace GG, Shepherd RK, Clark GM, O’Leary SJ. Polypyrrole-coated electrodes for the delivery of charge and neurotrophins to cochlear neurons. Biomaterials. 2009;30:2614–24.
Borre ED, Kaalund K, Frisco N, Zhang G, Ayer A, Kelly-Hedrick M, Reed SD, Emmett SD, Francis H, Tucci DL, et al. The impact of hearing loss and its treatment on health-related quality of life utility: a systematic review with meta-analysis. J Gen Intern Med. 2023;38:456–79.
Yeo BSY, Song H, Toh EMS, Ng LS, Ho CSH, Ho R, Merchant RA, Tan BKJ, Loh WS. Association of hearing aids and cochlear implants with cognitive decline and dementia: a systematic review and meta-analysis. JAMA Neurol. 2023;80:134–41.
Kim ES, Gustenhoven E, Mescher MJ, Pararas EE, Smith KA, Spencer AJ, Tandon V, Borenstein JT, Fiering J. A microfluidic reciprocating intracochlear drug delivery system with reservoir and active dose control. Lab Chip. 2014;14:710–21.
Chen H, Zhao Y, Li J, Guo M, Wan J, Weitz DA, Stone HA. Reactions in double emulsions by flow-controlled coalescence of encapsulated drops. Lab Chip. 2011;11:2312–5.
Guo J, Yu Y, Cai L, Wang Y, Shi K, Shang L, Pan J, Zhao Y. Microfluidics for flexible electronics. Mater Today. 2021;44:105–35.
Shang L, Yu Y, Gao W, Wang Y, Qu L, Zhao Z, Chai R, Zhao Y. Bio-inspired anisotropic wettability surfaces from dynamic ferrofluid assembled templates. Adv Funct Mater. 2018;28:1705802.
Ye B, Rong F, Gu H, Xie Z, Cheng Y, Zhao Y, Gu Z. Bioinspired angle-independent photonic crystal colorimetric sensing. Chem Commun (Camb). 2013;49:5331–3.
Chen Z, Kujawa SG, McKenna MJ, Fiering JO, Mescher MJ, Borenstein JT, Swan EE, Sewell WF. Inner ear drug delivery via a reciprocating perfusion system in the Guinea pig. J Control Release. 2005;110:1–19.
Tandon V, Kang WS, Robbins TA, Spencer AJ, Kim ES, McKenna MJ, Kujawa SG, Fiering J, Pararas EE, Mescher MJ, et al. Microfabricated reciprocating micropump for intracochlear drug delivery with integrated drug/fluid storage and electronically controlled dosing. Lab Chip. 2016;16:829–46.
Zhu Y, Kong B, Liu R, Zhao Y. Developing biomedical engineering technologies for reproductive medicine. Smart Med. 2022;1:e20220006.
Zhang H, Chen G, Yu Y, Guo J, Tan Q, Zhao Y. Microfluidic printing of slippery textiles for medical drainage around wounds. Adv Sci (Weinh). 2020;7:2000789.
Creff G, Bernard-Le Liboux N, Coudert P, Bourdon H, Pean V, Wallaert N, Lambert C, Godey B. Tonotopic and default frequency fitting for music perception in cochlear implant recipients: a randomized clinical trial. JAMA Otolaryngol Head Neck Surg. 2024;150:960–8.
van Heteren JAA, Wendrich AW, Peters JPM, Grolman W, Stokroos RJ, Smit AL. Speech perception in noise after cochlear implantation for single-sided deafness: a randomized clinical trial. JAMA Otolaryngol Head Neck Surg. 2025;151:211–9.
Vogel A, Saborowski A, Wenzel P, Wege H, Folprecht G, Kretzschmar A, Schütt P, Jacobasch L, Ziegenhagen N, Boeck S, et al. Nanoliposomal irinotecan and fluorouracil plus leucovorin versus fluorouracil plus leucovorin in patients with cholangiocarcinoma and gallbladder carcinoma previously treated with gemcitabine-based therapies (AIO NALIRICC): a multicentre, open-label, randomised, phase 2 trial. Lancet Gastroenterol Hepatol. 2024;9:734–44.
